Isolation, characterization and evaluation of antimicrobial activity of Withanolide-A of Withania somnifera


Ashish Ranjan Singh, Pratap Chand Mali





The increasing prevalence of multidrug resistant strains of bacteria and the recent appearance of strains with reduced susceptibility to antibiotics raises the specter of untreatable bacterial infections and adds urgency to the search for new infection-fighting strategies. The WHO estimated that approximately 80% of world population relies mainly on traditional medicines, mostly plant drugs in their health care. About 30% of the currently used therapeutics is of natural origin. Withania somnifera Dunal is widely used in Ayurvedic medicine, the traditional medical system of India. The alkaloid (Withanolide –A) was obtained from 50% ethanol extract of  Withania somnifera stem, subjected to column chromatography on silica gel eluted with a stepwise gradient of increasing order (Ethyl acetate: hexane). White crystals in ethyl acetate were obtained from ethyl acetate: hexane fraction. The aim of the present study was to investigate the in vitro antibacterial activity of the alkaloid, was screened against pathogenic bacteria of clinical origin including two species, Pseudomonas and Staphylococcus aureus.  The growth inhibition was observed in four of the five tested pathogens at three different of Withanolide-A concentrations (100, 500, and 1000 ppm).The result indicates that the Withanolide–A exhibited significant antibacterial activity against the Pseudomonas and Staphylococcus aureus.




Withania somnifera, Withanolide-A, Pseudomonas, Staphylococcus aureus


Full Text:



Article Metrics

Metrics Loading …

Metrics powered by PLOS ALM


  • There are currently no refbacks.


Copyright (c) 2014 International Journal of Pharmacological Research

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Dr. Jun Ren is a dedicated and experienced registered dietitian and nutritionist who is committed to helping people achieve their health goals through personalized nutrition plans. With a passion for promoting healthy eating habits and preventing chronic diseases, Dr. Ren has been able to assist numerous clients in improving their overall quality of life.

Leave a Comment