Primary health center, urban health center and district hospital rely on Sahali method for hemoglobin estimation. Sahali acid hematin method is still in vogue used, in spite of availability of more reliable and accurate methods. Sahali acid hematein method though effective and easy is not sufficiently accurate because it shows wide scatter in inter observers and intra observer result distribution. For good ethical laboratory practices it needed to develop the variability accuracy precision of hemoglobin estimation by Sahali acid hematin method compared to Drabkin cyanmethemoglobin method as per guidelines by ICMR. Hence the present study was undertaken for standardizing the hemoglobin result in district hospital laboratory. Objectives: Observe the inter-observational result variation using the Sahali method of Hemoglobin estimation and to validate the accuracy and precision of hemoglobin estimation by Sahali method as compared to Drabkin method. Material and Methods: This is a prospective hospital based study. 51 subjects from OPD were included in the study and duration of the study was 2 months. Sample for hemoglobin estimation was collected from the subjects in 3 different bottles and divided into group A, B and C according to the method of estimation. Results: Sahli’s method of estimation in the samples by two different groups of workers showed a significant difference. Comparison of haemoglobin estimated by Sahli’s and Drabkin method by finger prick and venepuncture method of collection not showed a significant difference.
: 0 | PDF
Copyright (c) 2014 International Journal of Biomedical and Advance Research
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work’s authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).